Membrane-bound Inorganic Pyrophosphatase of Human Erythrocytes.
نویسندگان
چکیده
منابع مشابه
Purification and some properties of inorganic pyrophosphatase from human erythrocytes.
Inorganic pyrophosphatase was purified BOO-fold from human erythrocytes by a procedure involving complete hemolysis of the cells, removal of hemoglobin, ammonium sulfate fractionation, diethylaminoethyl cellulose column chromatography, and gel filtration. Magnesium chloride and 2-mercaptoethanol stabilized the activity. The procedure yielded an enzyme with an optimal pH of 7.7, and an apparent ...
متن کاملCharacterization of plasma membrane bound inorganic pyrophosphatase from Leishmania donovani promastigotes and amastigotes.
BACKGROUND Currently, a major problem in the management of visceral leishmaniasis or kala-azar, especially in the Indian subcontinent, is the growing unresponsiveness to conventional antimonial therapy. Membrane bound pyrophophatase (PPases) do not exist in plasma membrane from mammals. Thus, H(+)-PPases from Leishmania plasma membrane might be potential target in rational chemotherapy of the d...
متن کاملYeast Inorganic Pyrophosphatase
The kinetics of the Mg2+ activation of crystalline yeast inorganic pyrophosphatase have been thoroughly investigated from pH 7.40 to pH 9.05 using a sensitive isotope assay in order to determine the role of the divalent metal ion activator in the reaction. A computer program has been devised for calculating the concentration of each of the various components of the complex equilibrium involving...
متن کاملYeast Inorganic Pyrophosphatase
The magnesium ion-dependent hydrolysis of pyrophosphate catalyzed by yeast inorganic pyrophosphatase is markedly inhibited by calcium ion. The kinetics of inhibition have been measured and analyzed in terms of a model involving strong interactions between CaPPi and two metalcomplexed forms of the enzyme, EMg and ECa. Inhibition constants for these interactions have been estimated by nonlinear r...
متن کاملPurification of yeast inorganic pyrophosphatase.
An enzyme which hydrolyzes inorganic pyrophosphate (PP) to orthophosphate (ortho P) was demonstrated by Lohmann (1) in muscle. Bauer (2) found that autolysates of slowly dried bottom yeast were a rich source of inorganic pyrophosphatase and were free of adenosinetriphosphatase (ATPase) activity. An important contribution was made by Bailey and Webb (3) who accomplished an extensive purification...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1971
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.25-1457